• Product Name4X Viral Lysis Buffer
• Brief DescriptionReagent
• ApplicationsWB;Chemiluminescent ELISA
• Concentration 4X
• Storage Stable at room temperature for at least 1 year.

• Tested and optimized for Western blotting and ELISA applications; Ready-to-use solution; Effective for extracting nuclear, cytosolic and membrane proteins; Prevent proteolysis and loss of PTMs without the need to add protease or phosphatase inhibitors

Completely lysis of the SARS-CoV-2 and the host cells in human specimens is the first step for the downstream immunoassays. GenuIN? 4x Viral Lysis Buffer is proprietarily formulated to lyse COVID-19 patients biospecimens or SARS-CoV-2 viruses from cultured cells.

• Antibody reactivity and specificity test. Serial dilutions of recombinant SARS-CoV-2 N protein (Cat# VPN2) (A), 3 positive and 2 negative human nasopharyngeal swab specimens (20 μL) (B), and different concentrations of gamma-irradiated SARS-CoV-2 (nCoV, B&C), SARS-CoV (SARS) (C), and MERS (C) were in Viral Lysis Buffer (Cat# VL101), and blotted with rabbit monoclonal anti-N primary antibody (Cat# VY7N, 1:2,000 dilution) and HRP-conjugated highly cross-adsorbed goat anti-rabbit IgG secondary antibody (Cat# 202), respectively. The blot was then incubated using PiQTM ECL Substrate Kit (Cat# 639) and imaged using a chemiluminescence digital imager. Note that 1) NP Swab POS No.3 has very low viral load; 2) There were no cross-reactivity of the antibody with either SARS or MERS.
• SARS-CoV-2 chemiluminescent ELISA using SARS-CoV-2. Different concentrations of gamma-irradiated SARS-CoV-2 viruses were lysed in Viral Lysis Buffer (Cat# VL101) and coated (150 μL) on a microplate. Rabbit monoclonal anti-N primary antibody (Cat# VYN7, 1:10,000 dilution) and HRP-conjugated highly cross-adsorbed goat anti-rabbit IgG secondary antibody (Cat# 202, 1:20,000) were used to detect SARS-CoV-2 N protein. PiQTM ECL (Cat# 636) reagents were used to generate chemiluminescent signals. RLU, relative light unit. ΔRLU = RLU of the well with primary antibody - RLU of the corresponding well without primary antibody, all else being equal.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting